Table 3.
Effect of re-vitrification in different stages on mouse embryonic development.
| Group | Fresh embryos (n) | No. of surviving embryos (%) | No. of re-vitrified embryos | No. of surviving re-vitrified embryos (%) | No. of expanded blastocysts (%) | No. of hatching blastocysts (%) |
|---|---|---|---|---|---|---|
| 1 | 100 | – | – | – | 100 (100.00) | 98 (98.00%) |
| 2 | 96 | 94 (97.92%) | – | – | 93 (98.94%) | 90 (96.77%) |
| 3 | 111 | 108 (97.30%) | – | – | 108 (100.00) | 99 (91.67%) |
| 4 | 102 | 102 (100.00%) | 102 | 102 (100.00%) | 100 (98.04%) | 98 (98.00%) |
| 5 | 104 | 104 (100.00%) | 104 | 100 (96.15%) | 96 (96.00%) | 86 (89.58%)* |
Group 1: Fresh embryos; Group 2; 8-cell vitrified embryos; Group 3; Blastocyst vitrified embryos; Group 4; Vitrified at the 8-cell stage and re-vitrified at 8-cell stage; Group 5; Vitrified at the 8-cell stage and re-vitrified at the blastocyst stage. *Significant difference with group 1 (P < 0.05).