Table 1.

Identified glucagon fragments (m/zs) and corresponding amino acid sequences

Calculated m/z	Observed m/z	Amino acid sequence	ID	Bond break
3482.8	3481.4	HSQGTFTSDYSKYLDSRRAQDFVQWLMNT	Gcg1-29	Native glucagon
2462.6	2461.3	HSQGTFTSDYSKYLDSRRAQD	Gcg1-21	Asp21-Phe22
2219.4	2220.3	HSQGTFTSDYSKYLDSRRA	Gcg1-19	Ala19-Gln20
2148.3	2148.3	HSQGTFTSDYSKYLDSRR	Gcg1-18	Arg18-Ala19
1738.9	1738.4	TFTSDYSKYLDSRR	Gcg5-18	Gly4-Thr5, Arg18-Ala19
1520.6	1520.4	HSQGTFTSDYSKY	Gcg1-13	Tyr13-Leu14

Abbreviation: ID, identification.