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Fig. 7. The strategy of activatable probes from NIR-I to NIR-II imaging: (a) rational molecular design of fluorescent probes (EW-BOD-SH) from NIR-I to NIR-II by introducing an enhanced electron-withdrawing group. (b) Self-immolative pathway of enzyme-activated probes. (c) The chemical structure of the NIR-I probe (BOD-K-βgal) and NIR-II probe (BOD-M-βgal). (d) NIR-I and NIR-II imaging of living mice.