Scheme 3. (A) Sequence of target MUC1 VNTR trimer 9 (ligation sites indicated by dotted lines); (B) synthesis of MUC1 VNTR bifunctional fragment 12via a side chain anchoring strategy; (C) synthesis of MUC1 VNTR trimer 9via one-pot iterative DSL reactions, PG = amino acids were side chain protected in resin-bound peptide; (D) HPLC trace and MALDI-TOF MS (inset) of purified MUC1 VNTR trimer 9; (E) ESI-MS of purified MUC1 VNTR trimer 9.
