Fig. 2. Investigation of α-fuc expression in canonical cellular senescence models. (A) Schematic illustration for generating replicative fibroblast cells and stress-induced premature senescent HCT116 cells. (B) Western blotting of p53, p21 and p16 in early and late fibroblast cells or control and senescent HCT 116 cells. (C) Chromogenic X-fuc assay of early and late fibroblast cells or control and senescent HCT 116 cells. (D) Real-time PCR analysis of FUCA1, GLB1, and GUSB mRNAs in early and late fibroblast cells or control and senescent HCT 116 cells. Data are represented as mean ± SEM (n = 3 in D). Statistical significance was determined by a two-way ANOVA test with a post hoc Bonferroni test. Different letters (e.g., a–d) signify datasets that are statistically distinct (p < 0.05).