Fig. 4. ALP mediated transient co-assembly of 1/ATP. (A) Fluorescence intensity of 1/ATP at 484 nm versus hydrolysed time with different concentrations of ALP at 298 K. Inset of (A): fluorescence spectral variations of 1/ATP versus hydrolysed time in the presence of 0.32 U mL⁻¹ of ALP. λ_ex = 420 nm. (B) Reversible emission color transition of 1 upon successive addition of ATP and ALP. (C) DLS data showing the induced assembly of 1 by ATP and disassembly by 0.32 U mL⁻¹ of ALP. (D) CD spectral variations of 1/ATP versus hydrolysed time in the presence of 0.32 U mL⁻¹ of ALP. (E) Fluorescence intensity of 1/ATP at 484 nm versus hydrolysed time with 0.32 U mL⁻¹ of ALP at varying temperature. (F) Time-dependent fluorescence intensity at 484 nm upon several repetitive additions of ATP to 1 in the presence of 0.11 U mL⁻¹, 0.23 U mL⁻¹, 0.41 U mL⁻¹ and 0.57 U mL⁻¹ of ALP, respectively. Arrows represent the (re)fueling points with the addition of ATP. ([1] = 2.0 × 10⁻⁵ M, [ATP] = 2.0 × 10⁻⁵ M, aqueous HEPES buffer, and pH = 7.0).