Figure 2. MADM labeling pattern in different organs and stem cell niches.

(A) Overview of MADM labeling (green, GFP; red, tdT; yellow, GFP/tdT) in MADM-19^GT/TG in combination with Hprt-Cre at P21. Diverse tissues/organs including eye, brain, lung, spinal cord, kidney, spleen, liver, heart, and thymus are illustrated.

(B) Schematic (left) and MADM labeling (middle/right; green, GFP; red, tdT; yellow, GFP/tdT) in mammary gland of lactating MADM-19^GT/TG;Hprt^Cre/+ female at 4 months of age. Basal/myoepithelial (middle) and luminal (right) cells are stained with antibodies against K14 and K8 (white), respectively.

(C) Schematic (left) MADM labeling (right; green, GFP; red, tdT; yellow, GFP/tdT) in MADM-19^GT/TG;Hprt^Cre/+ pancreas, acinus, and duct, at P21. Epithelial cells are visualized by antibody staining against b-catenin (white; b-Cat). Acinar cells are identified by the presence of intracellular secretory granules.

(D) Schematic (left) and MADM labeling (middle/right; green, GFP; red, tdT; yellow, GFP/tdT) in telogen (middle) and anagen (right) hair follicles in MADM-19^GT/TG;Hprt^Cre/+ at P21 (telogen) and P28 (anagen). Bu, bulge; 2° HG, secondary hair germ; SG, sebaceous gland; IRS, inner root sheath; CP, companion layer; ORS, outer root sheath; Mx, matrix.

(E) Schematic (left) and MADM labeling (right; green, GFP; red, tdT; yellow, GFP/tdT) in small intestine in MADM-19^GT/TG;Hprt^Cre/+ at P21. Epithelial cells are visualized by antibody staining against β-catenin (white; β-Cat). Asterisk marks a Paneth cell, identified by the presence of intracellular granules. TAC, transit-amplifying cell; LGR5, leucine-rich repeat-containing G-protein coupled receptor 5. Nuclei were stained using DAPI. Scale bar: 50 μm (A) and 20 μm (B–E).

See also Figures S5–S8.