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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Cell Rep. 2021 Jun 22;35(12):109274. doi: 10.1016/j.celrep.2021.109274

Figure 6. Sister chromatid segregation patterns based on YGRI in Dnah11 knockout (KO) and in different somatic cell lineages.

Figure 6.

(A) (Left) Summary of YGRI analysis in selected MADM reporters with Dnah11 depletion (in iv mice). (Right) YGR index for cortical projection neurons in P21 neocortex in MADM-7, MADM-12, and MADM-18 reporter lines in combination with the Emx1-Cre driver in control and Dnah11 KO (iv) mice. Note that a decrease of YGRI to 1 would indicate random sister chromatid segregation but that the YGRI was not decreased upon Dnah11 mutation. Bars represent mean ± SEM. Data show n = 3 mice for each genotype.

(B) Representative confocal microscopy images at P21 with MADM labeling (GFP, green; tdT, red) from selected MADM reporters in combination with a Nestin-Cre (cerebellum) or Emx1-Cre (cerebral cortex and hippocampal CA1 area) driver used for quantifications in Figures 5C and 6A. Arrows indicate Purkinje cells (Pcs), cortical pyramidal neurons (Pys), and CA1 pyramidal neurons (CA1 Pys). Scale bar: 100 μm.

(C) YGRI for selected MADM reporters in different neuronal lineages at P21. YGRI of cortical astrocytes and hippocampal CA1 pyramidal cells derived from Emx1+ progenitors and cerebellar Purkinje cells derived from Nestin+ progenitors significantly differ from the YGRI of cortical pyramidal neurons for most MADM chromosomes analyzed. Values represent mean ± SEM. Data show pyramidal neurons (n = 6); cortical astrocytes (n = 6); CA1 pyramidal neurons M5, M7, M8, M11, M12, M16, M18, and M19 (n = 6); M10 (n = 7); M17 (n = 8); cerebellar Purkinje cells M7, M8, M11, M16, M17, and M19 (n = 3); M12 and M18 (n = 4); M10 (n = 5) and M5 (n = 6 mice).

(D) (Left) White blood cell preparations from spleen in MADM reporters with Hprt-Cre at P21 were subjected to FACS. The number of green GFP+, red tdT+, and yellow GFP+/tdT+ CD3+ T cells (black) and CD19+ B cells (blue) were quantified. (Right) YGRI for six different MADM chromosomes including sparse (MADM-4), intermediate (MADM-8, MADM-15, and MADM-17), and dense (MADM-18 and MADM-19) lines. The different MADM recombinant chromosomes displayed distinct YGRI but YGRI for T cells and B cells was not significantly different for all MADM chromosomes analyzed. Bars represent mean ± SEM. Data show M8 and M18 (n = 3); M4, M17, and M19 (n = 4); M15 (n = 5 mice). Welch’s unequal variances t test, pM4 = 7.5E01, pM8 = 7.9E01, pM15 = 7.7E01, pM17 = 6.4E01, pM18 = 9.8E01, pM19 = 5.0E01.