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. 2021 Jul 14;12:708672. doi: 10.3389/fpls.2021.708672

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Copyright © 2021 Wang, Zhang, Ai, Wu, Li, Zhang and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Patch-clamp analysis of Ca²⁺-permeable channels in wild-type, cngc6, rbohB/D, cngc6/rbohB/D, cngc6/35S::RbohB-1, and cngc6/35S::RbohD-1 seedlings. The Ca²⁺ current before HS (at 22°C, control) and after HS (at 37°C, HS) was compared in the root cell protoplasts of 8-day-old wild-type (A), cngc6 (B), rbohB/D (C), cngc6/rbohB/D (D), cngc6/35S::RbohB-1 (E), and cngc6/35S::RbohD-1 (F) plants. The Ca²⁺ current was recorded by step voltage clamp. Each trace is a representative current from six protoplasts. Currents in the protoplasts are shown in the left and middle columns, respectively. The I–V curve is shown in the right column (mean ± SD, n = 6).