Figure 8.

Effects of CNGC6 via H₂O₂ on AtHSP17.7 and AtHSP21 expression. (A,B) About 10-day-old seedlings grown at 22°C were exposed to 37°C (HS) or maintained at 22°C (Control) for 2 h. Total protein was then extracted, separated by SDS-PAGE, and analyzed by Western blotting. Tubulin was used as an internal control. Three independent experiments were carried out; the results indicate similar trends in protein accumulation. (C,D) The relative HSP17.7 (C) and HSP21 (D) level after HS treatment. The data presented are the means ± SE of measurements taken from three independent experiments and represents the relative intensity of each signal. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 vs. Col (Student’s t-test).