Figure 1.

Ex-vivo phenotypic signatures of peripheral blood leukocytes from T. cruzi-infected cynomolgus macaques classified according to histopathological features of chronic chagasic cardiopathy. The phenotypic signatures were constructed based on the proportion of subjects in each cell subpopulation with biomarker levels above the global median cut-off, calculated from data from the entire study population. Ascendant curves were assembled from non-infected controls (NI) to draw the reference curves for innate and adaptive immunity cells, used for comparative analysis of results from T. cruzi-infected cynomolgus macaques. Data are displayed by bar charts (NI = ) and continuous ascendant lines (NI = ○). The T. cruzi-infected macaques were classified according to histopathological features of cardiac biopsies and referred to as CCC(–) for absence of chronic chagasic cardiopathy (); CCC(+) for mild chronic chagasic cardiopathy () and CCC(++) for moderate chronic chagasic cardiopathy (). Comparative analyses between T. cruzi-infected subgroups and non-infected controls were carried out considering the ascendant biomarker signature of non-infected controls as the reference curve. The differences between groups were considered for biomarkers with proportions confined to distinct 50^th percentiles as compared to the reference curve. The biomarkers with lower frequencies in T. cruzi-infected macaques were underscored by # and bold underline format. Those biomarkers with higher frequencies in T. cruzi-infected macaques were highlighted by *, bold underline format and color background.