Table 2.
Ex vivo cytokine profile of peripheral blood leukocytes from T. cruzi-infected cynomolgus macaques.
| Cell Phenotypes | Peripheral Blood | |||
|---|---|---|---|---|
| NI | CCC(-) | CCC(+) | CCC(++) | |
| Innate Immunity | ||||
| TNF+CD14+ | 24.6 ± 5.5 | 31.6 ± 4.7 | 31.9 ± 10.2 | 17.4 ± 6,6b |
| TNF+CD14+CD16+DR++ | 71.0 ± 46 | 60.8 ± 24.6 | 69.1 ± 7.8 | 61.8 ± 17.1 |
| TNF+CD16+ | 12.0 ± 5.1 | 13.1 ± 4.0 | 20.0 ± 8.2d | 7.6 ± 2.0 |
| IFN+CD16+ | 17.6 ± 5.3 | 14.7 ± 2.5 | 14.1 ± 4.5 | 14.7 ± 3.1 |
| IL-10+CD14+ | 31.3 ± 8.6 | 27.0 ± 8.2 | 24.4 ± 3.4 | 22.2 ± 4.8 |
| IL-10+CD14+CD16+DR++ | 29.8 ± 8.2 | 27.1 ± 1.1 | 14.2 ± 2.5b,d | 37.5 ± 7.8 |
| Adaptive Immunity | ||||
| TNF+CD4+ | 6.8 ± 1.4 | 7.5 ± 1.9 | 4.5 ± 1.6 | 7.0 ± 1.5 |
| TNF+CD8+ | 4.9 ± 1.0 | 4.5 ± 0.7 | 3.0 ± 1.0 | 6.6 ± 1.0c |
| TNF+CD20+ | 7.6 ± 1.4 | 7.3 ± 1.8 | 7.3 ± 2.2 | 14.5 ± 9.6a |
| IFN+CD4+ | 4.8 ± 0.7 | 9.0 ± 1.3 a | 8.3 ± 1.3a | 10.4 ± 2.2a |
| IFN+CD8+ | 5.0 ± 1.0 | 2.8 ± 0.7 | 3.5 ± 1.1 | 5.7 ± 2.2a,b |
| IL-10+CD4+ | 6.5 ± 2,4 | 11.1 ± 2.6 a | 17.0 ± 4.4a | 11.5 ± 2.6a |
| IL-10+CD8+ | 15.1 ± 2.9 | 9.7 ± 2.6 | 23.0 ± 4.6b | 32.1 ± 8.1a,b |
| IL-10+CD20+ | 25.0 ± 8.7 | 23.4 ± 3.0 | 24.7 ± 6.6 | 40.0 ± 14.9a |
NI, Non-infected macaques; CCC(-), Absence of Chronic Chagasic Cardiopathy; CCC(+), Mild Chronic Chagasic Cardiopathy; CCC(++), Moderate Chronic Chagasic Cardiopahty. Data are expressed as mean values (‰) ± standard error. Multiple comparisons amongst groups were carried out by Kruskal-Wallis test followed by Dunn’s post-test for sequential pairwise comparisons and significant differences at p<0.05 depicted by letters “a”, “b”, “c” and “d” as compared to NI, CCC(-), CCC(+) and CCC(++), respectively. All significant differences are highlighted in bold format.