Figure 14.
Burst experiment with P450BM-3 oxidation of lauric acid. One syringe of the stopped-flow spectrophotometer contained 4 μM P450BM-3 in the presence of 1 mM lauric acid and 50 mM potassium MOPS buffer (pH 7.5). The other syringe contained 100 μM NADPH in the same buffer. Final concentrations in the observation cell were 2 μM P450BM-3, 500 μM lauric acid, 50 mM potassium MOPS (pH 7.5), and 50 μM NADPH. Ten independent shots were averaged to yield the curve shown, with a measured rate of 608 ± 4 min−1 for the steady-state portion. In a separate experiment, the lauric acid was not included. No burst was observed.