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LC-HRMS of P450BM-3 reaction products. The traces were obtained 7 minutes after the reaction began with 25 nM P450BM-3, 100 μM tetradecyl sulfate, and 150 μM NADPH at room temperature. The samples were analyzed directly by LC-HRMS (negative ion mode). A. OH-tetradecyl sulfate channel (m/z 309.1741). B. Keto-tetradecyl sulfate channel (m/z 307.1585). C. Ketone plus OH tetradecyl sulfate channel (m/z 323.1534). D. Dihydroxy ((OH)2)-tetradecyl sulfate channel (m/z 325.169).
