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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: ACS Catal. 2020 Apr 2;10(9):5008–5022. doi: 10.1021/acscatal.0c00677

Figure 6.

Figure 6.

LC-HRMS of P450BM-3 reaction products following derivatization to form esters. A. Tetradecyl sulfate incubated with NADPH but without P450BM-3 for ~ 2 minutes. B. Tetradecyl sulfate incubated with NADPH and P450BM-3. Both reactions were followed by oxidation of alcohol products using Jones reagent47 and then Baeyer-Villiger ketone oxidation48 to form esters (Scheme 2). Extracted ion chromatograms for m/z 323.1534 are shown in Parts A and B, corresponding to a substrate ester derivative. C. HRMS (ESI−) mass fragmentation spectrum of the ester formed from incubation of tetradecyl sulfate with P450BM-3 and NADPH followed by the chemical oxidation steps.