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The percentage of apoptotic cells was examined with a NovoCyte flow cytometer. ABT-199 combined with HHT resulted in significant increases in the apoptosis rate in AML cell lines (A). The same results were observed in primary leukemia samples (B–F). The combination regimen exhibited minimal toxicity to normal peripheral blood mononuclear cells obtained from healthy donors (G) (*P < 0.05, **P < 0.01, and ***P < 0.001; NS, not significant).
