Table 1.
Primer names, size, and sequence used for targeting CpG sites for bisulfite sequencing.
| Primer Name | Length | CpG sites | Amplicon Size (bp) | Sequence |
|---|---|---|---|---|
| Forward 1 | 37 | 14 | 350 | GTTAGAYGAGGTGTTTAGGTAGTAGGATATTTGGTGG |
| Reverse 1 | 25 | 14 | 350 | TCCTCACCRCCCACTACCCACAAAC |
| Forward 2 | 29 | 9 | 328 | GGGTGAYGTGGTGTTATAGGTTTGTGTGG |
| Reverse 2 | 33 | 9 | 328 | AAAAAAAAACCRAACTTAAAAAAACAAACAATC |
| Forward 3 | 31 | 2 | 251 | TTATTYGTTAGAGTAGGTTATGGTAATTAGG |
| Reverse 3 | 38 | 2 | 251 | TAACCACRACCTCCCATACAAAATTAAATAATAAAAAC |
| Forward 4 | 29 | 12 | 309 | TYGAGGTATGTAGTAAAGTATTTGTGAAG |
| Reverse 4 | 34 | 12 | 309 | CTCTCCTAATCRAATTCATCAAAATAAACAAATC |
| Forward 5 | 37 | 6 | 320 | GTAATTTTGAGYGTGGTATGGTTGTTGGTGTTAGATG |
| Reverse 5 | 36 | 6 | 320 | TATAATACATCCRCCTCAAAATTATACATATTATAC |