Figure 5.

Anatomical and histological analysis of cerebellum in taiep rat at different stages of development. Representative images of T2-weighted coronal sections illustrating individual masks in cerebellum (yellow) at 1, 2, and 8 months (A). Scatter plot of the values of encephalic volume. WT encephalon volume was significantly bigger than that of taiep rats at 2 and 8 months (B). Non-significant differences were obtained for volume ratio cerebellum/encephalon of WT and taiep rats along the analyzed periods (C). The data obtained for each rat are represented by one point in the plot (at least n = 3 for group). Two-way ANOVA followed by Tukey's multiple comparison test was performed. Different letters indicate significant differences between groups (B,C). Bright-field micrographs of coronal slices of cerebellum of WT and taiep rat brains (10 months) (D). White dotted lines delimitate the white matter region in cerebellar folia. Myelin (red) and neurofilament (green) were revealed by immunofluorescence (E,F). An enlarged view of the white squares for merge and separated channels is shown in (F). Nuclei were revealed by DAPI (blue). Scale bars: 30 μm (E) and 10 μm (F). GL, granular layer; WM, white matter.