Figure 6.

Anatomical and histological matched analysis of corpus callosum in taiep and wild-type rats at different stages of development. Representative images of T2-weighted coronal sections illustrating individual masks in the brain structures of interest at 1, 2, and 8 months. Lateral ventricles in magenta and corpus callosum in green (A). The volume ratio was calculated as structure volume/encephalon volume (in mm³) at the same age (B,C). Scatter plot of the values of LV volume ratio (B) and CC volume ratio (C). Each dot represents the data from one rat (at least n = 3 for each group). Two-way ANOVA followed by Tukey's multiple comparison test was done. Different letters indicate significant differences between groups. Representative coronal slices of corpus callosum and striatum of WT and taiep rat brains (10 months) acquired by phase contrast microscopy (D). Myelin (red) and neurofilaments (green) were revealed by immunofluorescence (E–G). Amplifications of white squares for merge and separated channels are shown in (F). Striatum (G). Nuclei were revealed by DAPI (blue). Scale bars: 1,000 μm (D), 20 μm (E), 5 μm (F), and 100 μm (G). LV, lateral ventricle; CC, corpus callosum; Sr, striatum.