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. 2021 Jul 14;12:700958. doi: 10.3389/fpls.2021.700958

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Copyright © 2021 Huang, Kuo, Chuang, Yang, Huang, Jeng, Chen and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Temporal and spatial gene expression of TPS-b and TPS-e/f members in P. bellina. (A) Temporal and (B) spatial expression profiles of PbTPS5, PbTPS9, and PbTPS10. (C) Temporal and (D) spatial expression of PbTPS3 and PbTPS4. Temporal expression of PbTPS5, PbTPS9, and PbTPS10 was analyzed at floral bud stage (Bud), day of anthesis (Dd), and 3–17 days after anthesis (D + 3 to D17). Temporal expression of PbTPS3 and PbTPS4 was analyzed from 3 to 5 days before anthesis (D-5 and D-3) and day of anthesis (Dd) to 10 days after anthesis (D + 10) in P. bellina. Spatial gene expression of PbTPS genes was determined in various tissues, including sepal, petal, lip, column, stalk, leaf, and root. Data were obtained from three independent experiments. The expression of all genes was normalized to the reference gene, PbActin1 (Chuang et al., 2018b). Calculation of mean and standard error was based on triplicate repeats.