Figure 5.

Potential viroporin domains in the lagovirus (RHDV) non-structural proteins. Sequence analysis using PSIPRED secondary structure prediction tools (Buchan and Jones, 2019) revealed transmembrane (dark gray) and/or amphipathic pore-lining helices (light gray) in (A) p23 (pink) and (B) p29 (light blue); the MEMSAT-SVM algorithm (Nugent and Jones, 2012) was used for protein topology prediction; and Kyte-Doolittle plots indicate the hydrophobicity of amino acids. Note that the exact intracellular localization of p23 and p29 and the orientation of these proteins in cellular membrane(s) is currently unknown; if they localize to the ER, the term “cytoplasm” would indicate the lumen of the organelle.