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Optimization of the NaCl concentration for yielding the highest GAA production while maintaining Arabidopsis alg3 cell growth. (A) Effects of addition of 0–150 mM NaCl on the cell growth of a GAA-producing Arabidopsis alg3 cell culture. Data are average of three independent replications. (B,C) Evaluation of GAA accumulation in the NaCl-supplemented culture medium by GAA activity assay and Western blot, respectively. The media of 11-day-old cultures are used for the Western blot.
