Table 1.
Composition of the N-glycan structures in GAA produced in Arabidopsis alg3 cell cultures with and without NaCl supplementation.
| Abbreviation | Structure | Relative amount (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 95 kDa GAA | 76 kDa GAA | 95 kDa GAANaCl | 76 kDa GAANaCl | ||||||||
| N390 | N470 | N882 | N390 | N470 | N390 | N470 | N882 | N390 | N470 | ||
| M1 | ManGlcNAc2 | 8.7 | 1.5 | 21.2 | 4.4 | 1.4 | 14.7 | 1.2 | 33.0 | 30.1 | 1.6 |
| M2 | Man2GlcNAc2 | 6.4 | 9.5 | 10.1 | 11.4 | 9.5 | 10.2 | 7.6 | 11.3 | 13.2 | 11.2 |
| M3 | Man3GlcNAc2 | 68.0 | 68.2 | 51.1 | 67.5 | 75.5 | 56.6 | 57.8 | 51.8 | 54.0 | 80.1 |
| M4ER | Man4GlcNAc2 | – | – | – | 2.6 | 1.8 | 1.6 | 1.1 | – | 0.9 | 1.1 |
| M5ER | Man5GlcNAc2 | – | – | 1.5 | 6.6 | 4.5 | 1.4 | 3.1 | – | 0.6 | 2.3 |
| GlcM5ER | GlcMan5GlcNAc2 | – | – | – | 1.2 | 2.0 | – | 2.6 | – | – | – |
| GnM3 | GlcNAcMan3GlcNAc2 | 12.6 | 20.0 | 14.3 | 3.4 | 4.0 | 10.6 | 25.7 | 3.9 | 1.2 | 3.7 |
| Gn2M3 | GlcNAc2Man3GlcNAc2 | 2.7 | 0.8 | 1.8 | – | – | 2.2 | – | – | – | – |
| GalGnM3 | GalGlcNAcMan3GlcNAc2 | 1.6 | – | – | 2.9 | 1.3 | 2.0 | 0.9 | – | – | – |
| GalGn2M3 | GalGlcNAc2Man3GlcNAc2 | – | – | – | – | – | 0.7 | – | – | – | – |
| Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | |