Figure 12.

Recombination pattern outside of the OB in Lbhd2-CreERT2 mice. A, Confocal image at a sagittal plane ∼0.36 mm from the midline in a Lbhd2-CreERT2::Ai14 mouse. Scale bar, 1 mm. B, Coronal view at ∼1.46 mm posterior to the bregma, showing (Bi) labeled cells in the basolateral amygdaloid nucleus, fiber endings in the molecular layer medial amygdaloid nucleus and posterior piriform cortex; (Bii) densely labeled somata in the ventromedial nucleus of the hypothalamus; and (Biii) labeled fibers in the medial habenular nucleus. C, Summary of labeled structures with respect to distinct pathways. Top, MCs of the main OB are labeled, but not their cortical targets. Middle, Principal neurons of the accessory OB are labeled. Labeled fibers, but not somata, are visible in the medial amygdaloid nucleus. The target of the medial amygdaloid nucleus, namely, the ventromedial nucleus of the hypothalamus, has densely labeled cells. Bottom, Lateral septum densely contains labeled cells; the output fiber tracts are strongly labeled (stria medullaris), and labeled fibers are clearly visible in the target structure, namely, the medial habenular nucleus.