Figure 8.

Further characterization of tamoxifen-dependent recombination. A, Recombination pattern after 1× 160 mg/kg at P21, with tdTomato analyzed at P42. Scale bar, 0.1 mm. Bottom right, Summary of labeled soma positions relative to the EPL layers compared with Ra13-Cre::Ai14 and Lbhd2-CreERT2::Ai14 (3× 80 mg/kg). N = 3 mice. B, Recombination pattern following tamoxifen injection at P7. Lbhd2-CreERT2::Ai14 pups at postnatal day 7 were injected with a lowest dose tamoxifen (80 mg/kg intraperitoneally, once). Right, Recombination patterns from 2 females (F) and 2 males (M), as indicated. Scale bar, 0.5 mm. C, Tamoxifen-induced recombination with AAV-mediated expression. Tamoxifen (3× 80 mg/kg) was administered intraperitoneally starting on the day of AAV (AAV1-flex-EGFP) injection in the dorsal OB and EGFP expression analyzed 3 weeks later. AAVs for conditional expression can exhibit Cre-independent, “leak expression” depending on the production protocol (Fischer et al., 2019) or if not diluted enough.