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Diagram of experimental design. Skin fibroblasts were grown from a 1-year-old male rhesus monkey skin biopsy. This was followed by generating iPSCs which were differentiated using a cardiac differentiation protocol to derive the NHP iPSC-CMs for generation of the 2D monolayer and 3D EHT. Both culture conditions (2D, 3D) were then exposed to hypoxic conditions to allow transcriptional analysis by RNA-seq.
