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Design and fabrication of the master mold, PDMS mold, and NHP EHTs. (A) Master mold designed by AutoCAD and fabricated by 3D printing, and sizes in three dimensions are shown. (B) PDMS mold with flexible posts. (C) EHT formation in the PDMS mold at 30 min and 24 h. (D) Immunofluorescence staining of NHP EHTs at Day 50 with alpha-actinin (green), troponin T (red), and DAPI (blue) showing striated sarcomeres.
