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. 2020 Sep 1;117(9):2108–2124. doi: 10.1093/cvr/cvaa256

Figure 3.

Figure 3

Combination of HFD + ANGII increases left ventricular, atrial and pulmonary weights and increased myocardial cross-sectional area, and cardiac stress marker ANP. (A) LV/tibia, left ventricle weight corrected for tibia length (n = 7–10 mice per group). (B) Atria weight/tibia length (n = 6–11 mice per group). (C) Lung weight/tibia length (n = 7–10 mice per group). (D) Cross-sectional area of cardiomyocytes quantified by WGA-FITC staining (30–50 cells per animal, n = 6–9 mice per group). (E) Representative images of histological staining depicting the cardiomyocyte size (WGA), capillary density (CD31+), and fibrosis (Masson). (F) LV mRNA expression levels of ANP (n = 7–9 mice per group). ANP, atrial natriuretic peptide; CTRL, control chow; CTRL + ANGII, angiotensin II treated group on control chow; HFD, high-fat diet; HFD + ANGII, angiotensin II treated group on high-fat diet. Data are presented as mean + standard errors of the mean. *Kruskal–Wallis test followed by Mann–Whitney U test P <0.05 is considered significant.