. 2020 Sep 1;117(9):2108–2124. doi: 10.1093/cvr/cvaa256

Table 1.

Histology and RNA expression

	CTRL	HFD	CTRL + ANGII	HFD + ANGII
A) Histology	n = 8	n = 8	n = 7	n = 9
LV fibrosis (fold change)	1.0 ± 0.1	1.0 ± 0.1	1.4 ± 0.1	2.1 ± 0.2^a^,^b
Capillary density (number/mm²)	25.8 ± 1.3	25.4 ± 0.8	21.3 ± 1.2	18.8 ± 0.9^a
B) LV RNA expression levels	n = 8	n = 9	n = 7	n = 9
ANP	1.0 ± 0.3	1.0 ± 0.2	1.4 ± 0.2	2.8 ± 0.5^a^,^b
Col1a1	1.0 ± 0.1	1.8 ± 0.2	2.3 ± 0.3	2.7 ± 0.3^a^,^b
Col3a1	1.0 ± 0.2	1.6 ± 0.2	1.6 ± 0.1	2.6 ± 0.3^a^,^b
TIMP-1	1.0 ± 0.2	1.9 ± 0.4	3.3 ± 0.4	5.1 ± 0.9^a^,^b
Gal-3	1.0 ± 0.1	1.1 ± 0.1	1.2 ± 0.1	2.3 ± 0.3^a^,^b
IL-6	1.0 ± 0.5	0.3 ± 0.0	0.7 ± 0.1	2.4 ± 0.7

		HFD + ANGII + SALINE	HFD + ANGII + LIRA	HFD + ANGII + DAPA
C) LV RNA expression levels		n = 13	n = 13	n = 13
ANP		2.4 ± 0.5	0.9 ± 0.2^a	3.3 ± 0.5
Col1a1		2.6 ± 0.4	1.2 ± 0.2^a	2.5 ± 0.4
Col3a1		1.7 ± 0.1	1.1 ± 0.1^a	2.1 ± 0.2
TIMP-1		2.7 ± 0.5	1.3 ± 0.3^a	3.2 ± 0.4
Gal-3		2.0 ± 0.3	0.9 ± 0.2^a	1.4 ± 0.1
IL-6		2.9 ± 0.6	1.8 ± 0.4^a	2.8 ± 0.4

Data are presented as means ± standard error of the mean. (A) Histology. LV fibrosis = histological analysis of fibrosis by Masson staining. Capillary density is number of positive CD31 cells per mm². (B and C) LV RNA expression. Gene expression was normalized to 36B4 and presented as fold change to CTRL. Statistical testing was performed with Kruskal–Wallis test followed by Mann–Whitney U test.

ANP, atrial natriuretic peptide; Col1a1, collagen 1a1; Col3a1, collagen 3a1; CTRL, control chow; CTRL + ANGII, angiotensin II treated group on control chow; Gal-3, galectin-3; HFD, high-fat diet; HFD + ANGII, angiotensin II treated group on high-fat diet; HFD + ANGII + Dapa, high-fat diet + angiotensin II with dapagliflozin treatment; HFD + ANGII + Lira, high-fat diet + angiotensin II with daily liraglutide injection; HFD + ANGII + Saline, high-fat diet + angiotensin II with daily saline injections; IL-6, interleukin-6; TIMP-1, tissue inhibitor of metalloproteinases 1.

Compared to CTRL.

Compared to HFD.