Figure 1.
Lack of effect of Pdx on binding of P450cam and camphor. Spectra were recorded using 1.0 μM enzyme concentrations. (A) The estimated Kd values were 1.10 ± 0.20 μM in the absence of Pdx (black, ●) and 0.95 ± 0.12 μM in the presence of Pdx (red, ■). (B) The similarity of the difference spectra in the absence (black) and presence (red) of Pdx is shown for a concentration of 2.5 μM camphor.