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Lack of reversibility of P450cam binding spectra at high concentrations of camphor. (A) ΔA390-A420 as a function of camphor concentration. (B) Difference spectra of P450cam obtained with 40 μM (red) and 2 mM camphor (black). The P450cam concentration was 1.0 μM. The data points were not corrected for dilution.
