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. 2021 Jul 28;16(7):e0250807. doi: 10.1371/journal.pone.0250807

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© 2021 Nakagawa et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Urine concentrating capacity was assessed in male and female wildtype (Ren-b^+/+), heterozygous Ren-b knockout (Ren-b^+/-) and homozygous Ren-b knockout mice (Ren-b^-/-). Urine was first collected for 60 minutes post-hypotonic saline challenge (500 microliters of 0.03 mol/L NaCl; intraperitoneal). Subsequently, the same procedure was performed for a hypertonic saline challenge (500 microliters of 0.3 mol/L NaCl; intraperitoneal). *p<0.05 vs same genetic group on hypotonic challenge.