. 2020 Oct 10;128(1):1–16. doi: 10.1093/aob/mcaa181

Table 1.

Total root length and shoot dry mass of three root hair mutants (NRH = no root hair; BRH = bud root hair; SRH = short root hair) and the wild type (WT) in the ‘Optic’ background and in ‘Sassy’

Days from sowing	Soil	Genotype	Total root length 2017 (cm)		Shoot dry mass 2017 (mg)		Total root length 2018 (cm)		Shoot dry mass 2018 (mg)
24 (2017) and 19 (2018)	Sandy loam	NRH	71.5 ± 8.6^a		–		60.5 ± 4.7^a		24.3 ± 1.5^a
		BRH	79.3 ± 14.5^a		–		66.6 ± 6.5^a		25.9 ± 2.2^a
		SRH	66.9 ± 7.7^a		–		62.2 ± 3.4^a		22.0 ± 2.6^a
		WT	98.7 ± 11.8^a		–		66.1 ± 4.0^a		26.0 ± 1.5^a
		‘Sassy’	106.5 ± 8.7^a		–		78.3 ± 6.4^a		28.9 ± 1.0^a
	Clay loam	NRH	60.7 ± 5.9^a		–		35.9 ± 3.6^a		13.3 ± 1.9^a
		BRH	50.5 ± 2.9^a		–		51.7 ± 4.0^ab		18.8 ± 2.2^a
		SRH	49.4 ± 3.9^a		–		51.9 ± 1.6^ab		22.0 ± 2.6^a
		WT	68.3 ± 7.3^a		–		49.1 ± 1.8^ab		18.3 ± 1.6^a
		‘Sassy’	62.1 ± 3.7^a		–		56.9 ± 4.3^b		20.4 ± 1.3^a
33 (2018)	Sandy loam	NRH	–		–		347.1 ± 43.5^a		182.0 ± 15.8^a
		BRH	–		–		–		–
		SRH	–		–		–		–
		WT	–		–		393.5 ± 51.7^a		216.3 ± 41.0^a
		‘Sassy’	–		–		374.0 ± 57.4^a		213.9 ± 18.6^a
	Clay loam	NRH	–		–		173.2 ± 22.2^a		95.5 ± 36.0^a
		BRH	–		–		–		–
		SRH	–		–		–		–
		WT	–		–		277.0 ± 21.8^a		152.5 ± 40.1^a
		‘Sassy’	–		–		256.2 ± 27.5^a		157.3 ± 31.5^a
49 (2018)	Sandy loam	NRH	–		–		439.2 ± 51.0^a		509.4 ± 56.6^a
		BRH	–		–		716.8 ± 40.9^a		919.4 ± 169.3^ab
		SRH	–		–		428.0 ± 43.7^a		972.1 ± 125.4^ab
		WT	–		–		567.6 ± 46.3^a		802.3 ± 88.3^ab
		‘Sassy’	–		–		665.6 ± 58.6^a		1210.9 ± 31.1^b
	Clay loam	NRH	–		–		265.6 ± 23.2^ab		308.4 ± 43.2^a
		BRH	–		–		185.8 ± 17.0^a		560.0 ± 171.0^ab
		SRH	–		–		266.5 ± 18.3^ab		706.6 ± 226.2^ab
		WT	–		–		307.9 ± 24.5^ab		791.5 ± 79.9^b
		‘Sassy’	–		–		418.0 ± 35.7^b		826.3 ± 82.8^b
56 (2017) and 61 (2018)	Sandy loam	NRH	280.3 ± 106.9^a		111.5 ± 18.0^a		398.4 ± 50.3^a		1857.1 ± 779.6^a
		BRH	263.4 ± 73.1^a		182.8 ± 25.1^ab		–		–
		SRH	175.8 ± 66.4^a		176.0 ± 36.4^ab		–		–
		WT	95.6 ± 19.8^a		252.5 ± 25.2^b		663.6 ± 99.0^a		1579.6 ± 300.1^a
		‘Sassy’	99.1 ± 21.3^a		287.8 ± 48.5^b		735.8 ± 103.8^a		2088.3 ± 267.1^a
	Clay loam	NRH	58.7 ± 2.1^a		123.0 ± 15.9^a		269.4 ± 33.8^a		1223.9 ± 122.6^a
		BRH	116.2 ± 22.9^a		111.8 ± 12.8^a		–		–
		SRH	47.7 ± 7.6^a		150.8 ± 27.8^a		–		–
		WT	128.9 ± 5.9^a		127.3 ± 9.6^a		396.0 ± 34.4^ab		1761.3 ± 76.7^a
		‘Sassy’	103.9 ± 17.7^a		178.8 ± 67.7^a		514.2 ± 23.8^b		1863.6 ± 204.3^a
REML			F	P	F	P	F	P	F	P
Genotype			0.32	0.862	3.42	0.022	7.22	<0.001	4.33	0.003
Soil texture			4.44	0.045	8.98	0.006	59.48	<0.001	6.97	0.010
Time			6.84	0.014	–	–	64.61	<0.001	189.96	<0.001
Genotype × Soil texture			0.60	0.663	1.44	0.249	1.45	0.225	0.99	0.416
Genotype × Time			0.70	0.598	–	–	1.43	0.187	1.75	0.09
Soil texture × Time			1.70	0.202	–	–	5.67	0.001	1.26	0.291
Genotype × Soil texture × Time			1.11	0.370	–	–	0.69	0.701	0.75	0.644

Plants were grown in 2017 and 2018 at two locations with different soil textures: sandy loam and clay loam. Two sampling campaigns were done in 2017 at 24 and 56 d from sowing; four sampling campaigns were done in 2018 at 19, 33, 49 and 61 d from sowing. Data are the mean of four (2017) and eight replicates (2018), with differences between genotypes, soil textures and time of sampling established using REML for repeated measurements from which the F- and P-value data are derived. Significant parameters (P ≤ 0.05) are in bold. Identical superscript letters indicate no significant differences between genotypes as tested using one-way ANOVA followed by a post-hoc Tukey’s test. It should be noted that overall, the shoot biomass in 2018 (61 d from sowing) was approx. 10-fold of that recorded in 2017 (56 d from sowing) as a result of the respective sowing dates.