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. 2021 Jul 12;17(7):e1009663. doi: 10.1371/journal.pgen.1009663

Fig 5. Analysis of the recombination marker OLLAS::COSA-1 in the rmif-2 and rmh-1 mutants.

Fig 5

(A–C) Representative images (top) stained for DAPI (in magenta) and OLLAS (in green) in late pachynema (zone 7; defined in Fig 2) and quantification of OLLAS::COSA-1 nuclear foci (bottom) in the ollas::cosa-1, in ollas::cosa-1; rmif-2(jf113) and rmh-1(jf54); ollas::cosa-1 mutants. Scale bars, 10 μm. (D) Gonads were divided into seven equal zones from the mitotic tip to late pachynema (n = 3 gonads per genotype). Significant differences in foci distribution were determined using a Mann-Whitney test: zone 4: ollas::cosa-1 vs ollas::cosa-1; rmif-2 **** (p<0.0001); ollas::cosa-1 vs rmh-1; ollas::cosa-1 **** (p<0.0001); ollas::cosa-1; rmif-2 vs rmh-1; ollas::cosa-1 ns (p>0.9999). Zone 5: ollas::cosa-1 vs ollas::cosa-1; rmif-2 **** (p<0.0001); ollas::cosa-1 vs rmh-1; ollas::cosa-1 **** (p<0.0001); ollas::cosa-1; rmif-2 vs rmh-1; ollas::cosa-1 **** (p<0.0001). Zone 6: ollas::cosa-1 vs ollas::cosa-1; rmif-2 **** (p<0.0001); ollas::cosa-1 vs rmh-1; ollas::cosa-1 **** (p<0.0001); ollas::cosa-1; rmif-2 vs rmh-1; ollas::cosa-1 **** (p<0.0001). Zone 7: ollas::cosa-1 vs ollas::cosa-1; rmif-2 **** (p<0.0001); ollas::cosa-1 vs rmh-1; ollas::cosa-1 **** (p<0.0001); ollas::cosa-1; rmif-2 vs rmh-1; ollas::cosa-1 **** (p<0.0001).