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. 2021 Jul 12;17(7):e1009663. doi: 10.1371/journal.pgen.1009663

Fig 8. RMH-1 and RMIF-2 suppress heterologous recombination to different extents.

Fig 8

(A) A heterologous recombination assay [12] was used to determine the involvement of rmh-1 and rmif-2 in suppressing illegitimate recombination events. The method used to score heterologous recombination relies on the use of the mIn1 inversion on chromosome II (scoring for the exchange of shown genetic markers). (B) In WT (n = 2029 worms), no heterologous recombination was observed among the progeny; rmif-2 (n = 2018) 41 recombinant progeny; rmh-1 (n = 1090), 79 recombinant progeny. (C) Rate of heterologous recombinant progeny: WT, 0%; rmif-2, 2.3%; and rmh-1, 7.24%. The level of heterologous recombination in the rmh-1 mutant is around three times higher than in the rmif-2 mutant. Statistical analysis was done with a Fisher’s exact test: WT vs rmif-2 **** (p<0.0001); WT vs rmh-1 **** (p<0.0001); rmif-2 vs rmh-1 **** (p<0.0001).