Table 3. SNP positions and primers.
SNP identifier | SNP position | Primer pair |
---|---|---|
Chromosome IV | ||
A | -16 | Forward: 5′ CGCATAAATCCAACGTTCTCTG 3′ Reverse: 5′ AATCCATAAGTTTCGTGTTGG 3′ |
B | 1 | Forward: 5′ AAAATGGGAAGCGTACCAAA 3′ Reverse: 5′ TGCTTGTAGCGTTTCCAAGA 3′ |
C | 8 | Forward: 5′ GACACGACTTTAGAAACAACA 3′ Reverse: 5′ TGGTATGGAGTCCCTATTTTG 3′ |
D | 14 | Forward: 5′ GAATTTCAGGTGTTGGAAGG 3′ Reverse: 5′ TGCTCTGAAAAAATTGGCTG 3′ |
Chromosome V | ||
A | -17.5 | Forward: 5′ TTTCGGAAAATTGCGACTGT 3′ Reverse: 5′ CGCGTTTTGGAGAATTGTTT 3′ |
B | -5 | Forward: 5′ GAGATTCTAGAGAAATGGACACCC 3′ Reverse: 5′ AAAAATCGACTACACCACTTTTAGC 3′ |
C | 1 | Forward: 5′ AGAAATGATCCGATGAAAAAGC 3′ Reverse: 5′ CCGATAGTGTTCATAGCATCCC 3′ |
D | 5.8 | Forward: 5′ AGCCACATAAGCGCAATAAC 3′ Reverse: 5′ GTGACGGAACAAACTCATCTGC 3′ |
E | 17.8 | Forward: 5′ GCCACTGATGGGACAGAACC 3′ Reverse: 5′ CAGAAAATTGCCAAAACTACCG 3′ |