Fig. 3. The AH is required for KLC1E^CTD binding to liposomes.

(A) Representation of the His-tagged KLC1B/E^CTD constructs. (B) Cosedimentation analysis of His-KLC1B/E^CTD with Folch fraction I LUV or SUV. Samples were analyzed by SDS-PAGE/Coomassie (top) and quantified by densitometry (bottom). Means ± SEM of at least three experiments. **P < 0.01 and ***P < 0.001 compared to the sample without liposomes or as indicated in the graph. (C) Sequence comparison and visualization of the AH in the I560P KLC1E^CTD mutant. (D) Cosedimentation analysis of WT or I/P His-KLC1E^CTD with Folch fraction I LUV or SUV. Means ± SEM of at least three experiments. **P < 0.01 and ***P < 0.001 compared to the sample without liposomes or as indicated in the graph. (E) BS3 cross-linking assay showing oligomers of WT, but not I/P mutant His-KLC1E^CTD upon incubation with Folch fraction I LUV or SUV. (F) Cryo–electron microscopy observation of Folch fraction I LUV in the absence or presence of WT or I/P mutant KLC1E^CTD. Scale bar, 200 nm.