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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Gastroenterology. 2019 Apr 9;157(2):537–551.e9. doi: 10.1053/j.gastro.2019.04.003

Fig. 1. Mapping of HCV protein expression by time-resolved proteogenomics.

Fig. 1.

(A) Hepatocyte-like Huh7.5.1dif cells were infected with HCVcc (Jc1E2FLAG) over 10 days. Sampling intervals for transcriptomics, metabolomics and proteomics are indicated. (B) Quantification of HCV-specific peptides over the infection time course relative to non-infected and mock-infected (Mock) cells. The data represent TMT ratios with the 131 channels in the denominator (internal reference = mix of all samples). All ratios were normalized for the median of all 120,000 distinct peptides for each time point. The FLAG peptide is indicated by asterisk. (C) Increase of HCV protein abundance between 3–7 days pi. Data are displayed as relative expression (mean) of all peptides corresponding to a given HCV protein over the time course. HCV replication is depicted in red as median of total HCV peptide abundance ± standard error of the median.