Figure 5.

Inactivation of AKT signalling rescues the pro‐hypertrophic effects of MD1 deficiency in Ang II‐treated cardiomyocytes. (A) mRNA levels of hypertrophy markers in H9C2 cells treated with AdshMD1 and MK2206, in response to Ang II, were determined using qRT‐PCR. PBS was used as the control solution. (B,C) Representative blots and quantitative results showing the phosphorylation and total protein levels of AKT, mTOR and GSK3β following infection with AdshMD1, in PBS (B) and Ang II (C) treatments, in the absence or presence of MK2206. Cells infected with AdshRNA were used as controls. n = 3, P‐values are shown above the connection lines between compared columns.