Author response image 2. "line search” approach vs. TDLM on the simulated spiking data (assuming single ground-truth sequence).

(a) The rate map of the simulated place cells (n=40) over a linearized space with 80 positions. It is smoothed with 2 sample gaussian kernel, to mimic overlapping place fields. (b) We simulated a ground truth sequence with time lag of 2 time samples between successive firings in the ripple event. The histogram is the sequence distribution of the shuffled data (in blue). The red line is the sequence results for the true data. The permutation is done by shuffling the rate map of each cell, so that the place fields are randomized. (c) We randomly shuffled the firing order in the ripple event, so that there is no structured sequence in the simulation. We show that all methods report non-significant evidence of sequenceness.