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. 2021 Jul 1;58(1):2002978. doi: 10.1183/13993003.02978-2020

FIGURE 1.

FIGURE 1

Characterisation of MSC-derived extracellular vesicles (MSC-EVs). a) Characterisation of MSC-EVs using nanoparticle tracking analysis (histogram generated from five independent measurements). b) Representative transmission electron microscopy (TEM) images showing: multivesicular bodies (upper left, scale bar=50 nm), exosomes (bottom left, scale bar=100 nm), mitochondria like structures (upper right, scale bar=1 µm) and mitochondria like structures (bottom right, scale bar 200 nm). The images were taken using TEM microscope (JEOL, JEM 1400Plus, Japan). c) Immunoblot for protein expression levels of TOM20 in MSC-EVs and MSC-EVs-Rho lysates. All lanes were loaded with the same amount of total protein. d) Representative flow cytometry plots of MSC-EVs conjugated with 4 µm beads demonstrating presence of EVs populations double positive for CD44 and MitoTracker Deep Red FM (19.1%) and EV populations double positive for CD63 and MitoTracker Deep Red FM (23.8%). e) Representative histogram of nanoparticle tracking analysis of EVs isolated from Rhodamine-6G pre-treated MSC (histogram generated from five independent measurements). f) Immunoblot for protein expression levels of TOM20 in Rho-EVs lysates. All lanes were loaded with the same amount of total protein as in c.