Table 1.

Relative errors and repeatability of the Kalman-Excel method.

Sample name	Conc. ratios (HCT/LSP)	n	HCT			LSP
			C HCT (μg/mL)	RE (%)	RSD (1/2RSDH) (%)	C LSP (μg/mL)	RE (%)	RSD (1/2RSDH) (%)
M1	1 : 24	1	1.002	0.20	0.30	24.206	0.86	0.07
		2	0.996	−0.40	(8.00)	24.238	0.99	(4.96)
		3	0.999	−0.10		24.229	0.95
		Average	0.990	−0.10		24.224	0.93
M2	2 : 20	1	1.981	−0.95	0.175	20.115	0.57	0.05
		2	1.981	−0.95	(7.21)	20.112	0.56	(5.10)
		3	1.975	−1.25		20.131	0.66
		Average	1.979	−1.05		20.119	0.6
M3	3 : 16	1	2.982	−0.60	0.166	15.961	−0.24	0.06
		2	2.973	−0.90	(6.78)	15.971	−0.18	(5.27)
		3	2.974	−0.87		15.979	−0.13
		Average	2.976	−0.79		15.970	−0.18
M4	4 : 12	1	3.975	−0.62	0.14	12.067	0.56	0.02
		2	3.966	−0.85	(6.49)	12.071	0.59	(5.50)
		3	3.965	−0.87		12.072	0.60
		Average	3.969	−0.78		12.070	0.58
M5	5 : 08	1	5.008	0.16	0.20	8.019	0.24	0.10
		2	4.991	−0.18	(6.28)	8.035	0.44	(5.85)
		3	4.990	−0.20		8.031	0.39
		Average	4.996	−0.07		8.028	0.36
M6	6 : 04	1	5.921	−1.32	0.21	4.064	1.60	0.17
		2	5.897	−1.72	(6.11)	4.074	1.85	(6.49)
		3	5.904	−1.60		4.077	1.93
		Average	5.907	−1.55		4.072	1.79
M7	5 : 20	1	4.990	−0.20	0.15	20.203	1.02	0.06
		2	4.975	−0.50	(6.28)	20.224	1.12	(5.10)
		3	4.980	−0.40		20.220	1.10
		Average	4.982	−0.37		20.216	1.08

RSD_H: RSD value calculated from the Horwitz function; M1: mixture of HCT 1.000 μg/mL + LSP 24.000 μg/mL; M2: mixture of HCT 2.000 μg/mL + LSP 20.000 μg/mL; M3: mixture of HCT 3.000 μg/mL + LSP 16.000 μg/mL; M4: mixture of HCT 4.000 μg/mL + LSP 12.000 μg/mL; M5: mixture of HCT 5.000 μg/mL + LSP 8.000 μg/mL; M6: mixture of HCT 6.000 μg/mL + LSP 4.000 μg/mL; M7: mixture of HCT 5.000 μg/mL + LSP 20.000 μg/mL.