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. 2021 Jul 21;2021:5574130. doi: 10.1155/2021/5574130

Figure 1.

Figure 1

LINC00909 serves as a ceRNA of MRC2, LASP1 and ZNF839 by sponging miR-23b (a) Venn diagram showed the overlap of the miR-23b-3p's targets from miRcode, TargetScan and miRDB database. (b) The levels of putative targets of miR-23b-3p in SKOV3 were determined by real-time qPCR. miR-23b-3p was stably transfected into SKOV3 cells. (c-d) Dual-luciferase reporter assay verified the interaction between miR-23b-3p and its putative mRNA targets. C shows the sequences of binding sites of miR-23b-3p and its putative targets. 293T cells were transiently cotransfected with the indicated constructs. (e) Scatter plot showed the correlation between LINC00909 and MRC2/ZNF839/DEPC1/LASP1 mRNA in clinical specimens. R: Pearson correlation coefficient. (f) Real-time qPCR revealed mRNA level of MRC2/ZNF839/DEPC1/LASP1 in OVCAR4 cells. In OVCAR4 cells (in S4C), LINC00909 was stably transfected, and miR-23b-3p inhibitor was transiently transfected.