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. 2021 Jul 21;2021:5574130. doi: 10.1155/2021/5574130

Figure 6.

Figure 6

miR-23b-3p is a critical functional mediator of LINC00909. (a) Dual-luciferase reporter assay verified the interaction between miRNAs and LINC00909. 293T cells were transiently cotransfected with LINC00909-Firefly-luciferase construct and miRNA-mimics/negative control. N = 4. (b) Venn diagram showed the overlap between ovarian cancer expressed and LINC00909 binding miRNAs. (c) Colony formation assay revealed the tumor characteristics of each SKOV3 cell line. miRNA expression level was presented in S3B. Scale bar, 1 mm. (d) The level of LINC00909 was determined by real-time qPCR. OVCAR4 cells were stably transfected with LINC00909-mut (LINC-m, miRNA binding site mutant) or LINC00909 (LINC). (e) Colony formation assay revealed the tumor characteristics of each OVCAR4 cell line. Cells in S3D were used. Scale bar, 1 mm.