| Cycle number | Denature | Anneal | Extend | Final |
|---|---|---|---|---|
| 1 | 58 °C, 5 min | |||
| 72 °C, 5 min | ||||
| 2 | 98 °C, 45 s | |||
| 3–14 | 98 °C, 15 s | 63° C, 10 s | ||
| 15 | 72 °C, 1 min | |||
| 16 | 8 °C, hold |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.