Fig. 6. Effect of estrogen on the adipogenic differentiation of cADSCs. cADSCs were induced to adipogenic differentiation in MDIE and DRIE condition for 21 days. Oil Red-O staining images of (A) MDIE condition and (B) DRIE condition. Scale bar indicates 50 µm. Quantification of the stained lipid droplets of (C) MDIE condition and (D) DRIE condition. The results are presented as mean ± SEM. cADSC, canine adipose-derived stem cell; MDI, 3-isobutyl-1-methylxanthine + dexamethasone + insulin; DRI, dexamethasone + rosiglitazone + insulin; SEM, standard error of mean; MDIE condition, MDI, MDIE, and MDIE+ICI182,780; DRIE condition, DRI, DRIE, and DRIE+ICI182,780.

^*p < 0.001 vs. MDI or DRI, ^†p < 0.001 vs. MDIE or DRIE.