Fig. 8. Effect of estrogen on the mRNA gene expressions related to adipogenic differentiation of cADSCs. cADSCs were induced to adipogenic differentiation in MDIE and DRIE condition for 21 days. (A) FABP4, (B) LPL, and (C) LEPTIN mRNA expression in MDIE condition and (D) FABP4, (E) LPL, and (F) LEPTIN mRNA expression in DRIE condition were quantified by qRT-PCR.

cADSC, canine adipose-derived stem cell; FABP4, fatty acid-binding protein 4; LPL, lipoprotein lipase; qRT-PCR, quantitative real-time reverse-transcription polymerase chain reaction; MDI, 3-isobutyl-1-methylxanthine + dexamethasone + insulin; DRI, dexamethasone + rosiglitazone + insulin.

^*p < 0.05, ^†p < 0.01, ^‡p < 0.001 vs. MDI or DRI, ^§p < 0.05, ^∥p < 0.01, ^¶p < 0.001 vs. MDIE or DRIE.