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. 2021 Jul 22;16:4985–5002. doi: 10.2147/IJN.S319814

Table 1.

Experimental Runs, Independent Variables, and Measured Responses of the 31×41 Full Factorial Experimental Design of Epl-loaded MNLCs

F (Sorted by Standard Order) Type of Surface Modifier Concentration of Surface Modifier (% w:v) Y1: PS (nm) Y2: PDI Y3: ZP (mV) Y4: EE% (% w:w) Y5: Viscosity (cP) at Shear Rate (20 [1/s])
MNLC-1 Hydrogenated collagen 0.3 152.75±15.20 0.333±0.01 −28.1±0.42 62.945±1.10 39.24±0.1
MNLC-2 COSL 0.3 282.25±32.31 0.382±0.01 +15.8±0.85 69.948±0.77 0±0
MNLC-3 Hyaluronic acid 0.3 167±42.57 0.416±0.16 −44.25±1.63 81.285±1.63 186.39±0.32
MNLC-4 Hydrogenated collagen 0.5 155.6±4.10 0.362±0.07 −24.1±0.57 63.865±1.89 39.24±0.56
MNLC-5 COSL 0.5 236.15±26.38 0.428±0.06 +20.65±0.49 70.583±9.08 78.48±0.98
MNLC-6 Hyaluronic acid 0.5 262.75±84.07 0.449±0.03 −48.1±1.13 79.025±1.05 333.54±0.15
MNLC-7 Hydrogenated collagen 0.7 127.773±9.15 0.381±0.13 −27.95±0.21 67.281±8.39 49.05±0.17
MNLC-8 COSL 0.7 222.95±11.10 0.433±0.03 +29.3±0.28 70.26±0.08 29.43±2.11
MNLC-9 Hyaluronic acid 0.7 261.75±66.40 0.434±0.04 −53.2±1.13 88.445±2.80 824.04±1.65
MNLC-10 Hydrogenated collagen 0.9 161.2±5.23 0.393±0.04 −30.944±0.93 76.905±7.96 39.24±1.24
MNLC-11 COSL 0.9 397.1±158.11 0.428±0.16 +46.35±1.77 74.357±6.73 9.81±0.98
MNLC-12 Hyaluronic acid 0.9 277.45±36.56 0.475±0.01 −54.4±0.49 90.775±3.57 1373.4±0.47

Notes: Data presented as means ± SD (n=3). All systems contained 1.25 mg Epl/1 mL.

Abbreviations: PS, particle size; PDI, polydispersity index; ZP, ζ-potential; EE, entrapment efficiency.