Fig. 1.

Single-cell RNA sequencing revealed the complexity of SLE PBMC. (a) Pipeline of single cell RNA sequencing data processing. (b) A UMAP plot representing the 20 clusters across 258 868 PBMCs from eight individuals (five SLE patients and three healthy controls (HC)). (c) Violin plots showing expression of marker genes for 20 distinct cell types. (d) Heatmap showing expression of the top 3 DEGs in each cell type. (e) Bar plots showing the proportion of cell types in each sample. (f) Ration comparison of each cell type in SLE and HC groups. The red arrow represents significantly elevated cell types in the SLE group, while the blue arrow indicates the opposite. Data were analyzed using the Chi-Squared Test. * P < 0.01 (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).