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. 2021 Jun 25;297(2):100921. doi: 10.1016/j.jbc.2021.100921

Figure 6.

Figure 6

LigIIIα-TDP1 flexibility in solution.A, SEC-SAXS-MALS chromatographs for LigIII170–755- TDP1 and LigIIIα-TDP1. Solid lines represent the UV 280 nm (light blue) or SAXS signal (blue) in arbitrary units, while symbols represent molecular mass determined by MALS (cyan) and Rg values for the principal SEC peak (blue) versus elution time. SEC-SAXS-MALS results show that LigIII170–755-TDP1 is a homodimer and LigIII-TDP1 is heterotetramer. B, normalized P(r) functions of LigIII170–755-TDP1 and LigIIIα-TDP1. C, SAXS derived two-state model of LigIII170–755-TDP1 (top) and LigIIIα-TDP1 (bottom) are shown in comparison to the atomistic model derived by EM (shown in left panel). Weights of both SAXS conformers are indicated in percentage. Interaction between LigIII BRCT dimer (dark blue) and XRCC1 is highlighted. TDP1 and LigIII catalytic core are colored accordingly. Corresponding SAXS fits for the atomistic models are shown in Figure S8. Maximal dimension of the complexes (Dmax) determined from the P(r) functions (see panel B) is highlighted with the bars.